EMPIAR-10819
Benchmark FIB SEM data of HeLa cells previously imaged by Zeiss LSM900 Airyscan microscopy [multiple data sets in TIFF format]
Publication:

Raw and processed benchmark FIB SEM data matching Airyscan data deposited on Biostudies

Krentzel D, Laine RF, Domart MC, Peddie CJ, Henriques R, Collinson LM, Jones ML

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Related EMDB entry:
Deposited:
2020-08-14
Released:
2021-10-29
Last modified:
2021-10-29
Imageset size:
1.68 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
  • micrographs - multiframe
1. Raw FIB SEM run 1 part 1
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
1599
Image size:
(12032, 12032)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(50.0, 50.0)
Details:
First part of the raw data (out of 4 in total).
Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.
Files:
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2. Raw FIB SEM run 1 part 2
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
1600
Image size:
(12032, 12032)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(50.0, 50.0)
Details:
Second part of the raw data (out of 4 in total).
Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.
Files:
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3. Raw FIB SEM run 1 part 3
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
1534
Image size:
(12032, 12032)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(50.0, 50.0)
Details:
Third part of the raw data (out of 4 in total).
Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.
Files:
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4. Raw FIB SEM run 2
Category:
micrographs - single frame
Image format:
TIFF
No. of images or tilt series:
1261
Image size:
(12032, 12032)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(50.0, 50.0)
Details:
Last part of the raw data (out of 4 in total).
Images were acquired at 5 nm isotropic resolution throughout the region of interest, using a 6 us dwell time. During acquisition the SEM was operated at an accelerating voltage of 1.5 kV with 1.5 nA current.
Files:
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5. Aligned full resolution FIB SEM stack in its original orientation
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(7000, 965)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(50.0, 50.0)
Details:
Processed and aligned stack of FIB SEM images at 5 nm isotropic resolution in the original orientation of the acquisition
Files:
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6. Aligned full resolution FIB SEM stack resliced to match the orientation of the Airyscan data
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(5938, 7000)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(50.0, 50.0)
Details:
Processed and aligned stack of FIB SEM images at 5 nm isotropic resolution resliced to match the orientation of the Airyscan data available on Biostudies S-BSST707
Files:
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7. Downscaled (10nm) aligned FIB SEM stack resliced to match the Airyscan dataset
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(2969, 3500)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(100.0, 100.0)
Details:
Processed and aligned stack of FIB SEM images downscaled to 10 nm isotropic resolution and resliced to match the orientation of the Airyscan data available on Biostudies S-BSST707
Files:
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8. Downscaled (20nm) aligned FIB SEM stack resliced to match the Airyscan dataset
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(1485, 1750)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(200.0, 200.0)
Details:
Processed and aligned stack of FIB SEM images downscaled to 20 nm isotropic resolution and resliced to match the orientation of the Airyscan data available on Biostudies S-BSST707
Files:
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Files:
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