EMPIAR-10804 - electron cryo-tomograms of axons from human cerebral organoids, expressing GFP-ESYT1 [multiple data sets in MRC and TIFF formats]

EMPIAR-10804

electron cryo-tomograms of axons from human cerebral organoids, expressing GFP-ESYT1 [multiple data sets in MRC and TIFF formats]

Publication:

Electron cryo-tomography reveals the subcellular architecture of growing axons in human brain organoids

Hoffmann P, Giandomenico S, Ganeva I, Wozny M, Sutcliffe M, Lancaster M, Kukulski W

eLife 10 (2021)

DOI: 10.7554/eLife.70269

Related EMDB entry:

EMD-13197

Deposited:

2021-07-20

Released:

2021-11-01

Last modified:

2021-11-01

Imageset size:

40.89 GB

Imageset DOI:

10.6019/EMPIAR-10804

Experimental metadata:

Download xml json

Contains:

reconstructed volumes
micrographs - multiframe

Image Sets

1. reconstructed electron tomographic volumes of axons from cerebral organoids expressing GFP-ESYT1

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(1920, 1854)

Pixel type:

SIGNED BYTE

Pixel spacing:

(7.4, 7.4)

Details:

Tomograms were reconstructed with IMOD using back projection with a SIRT-like filter from stacks aligned by patch tracking, binning 2.

Files:

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2. Unaligned raw image frames of tilt series acquired on axons from cerebral organoids expressing GFP-ESYT1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(3.7, 3.7)

Details:

10 folders, each corresponding to one tilt series and containing raw image frames as well as metadata in an .mdoc file. The image frames are not aligned and not gain-corrected. An additional folder contains the gain reference and defect file.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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