EMPIAR-10804
electron cryo-tomograms of axons from human cerebral organoids, expressing GFP-ESYT1 [multiple data sets in MRC and TIFF formats]
Publication:

Electron cryo-tomography reveals the subcellular architecture of growing axons in human brain organoids

Hoffmann P, Giandomenico S, Ganeva I, Wozny M, Sutcliffe M, Lancaster M, Kukulski W

eLife 10 (2021)

Related EMDB entry:
Deposited:
2021-07-20
Released:
2021-11-01
Last modified:
2021-11-01
Imageset size:
40.89 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • reconstructed volumes
  • micrographs - multiframe
1. reconstructed electron tomographic volumes of axons from cerebral organoids expressing GFP-ESYT1
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
10
Image size:
(1920, 1854)
Pixel type:
SIGNED BYTE
Pixel spacing:
(7.4, 7.4)
Details:
Tomograms were reconstructed with IMOD using back projection with a SIRT-like filter from stacks aligned by patch tracking, binning 2.
Files:
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2. Unaligned raw image frames of tilt series acquired on axons from cerebral organoids expressing GFP-ESYT1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
10
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(3.7, 3.7)
Details:
10 folders, each corresponding to one tilt series and containing raw image frames as well as metadata in an .mdoc file. The image frames are not aligned and not gain-corrected. An additional folder contains the gain reference and defect file.
Files:
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