EMPIAR-10773 - Cryo-electron tomography of rod outer segments in wild type mice acquired conventionally with defocus [2 reconstructed volumes in MRC format]

EMPIAR-10773

Cryo-electron tomography of rod outer segments in wild type mice acquired conventionally with defocus [2 reconstructed volumes in MRC format]

Publication:

Determinants shaping the nanoscale architecture of the mouse rod outer segment

Pöge M, Mahamid J, Imanishi SS, Plitzko JM, Palczewski K, Baumeister W

eLife 10 (2021)

PMID: 34931611

DOI: 10.7554/elife.72817

Related EMDB entry:

EMD-13321 EMD-13322

Deposited:

2021-07-16

Released:

2022-01-07

Last modified:

2022-01-07

Imageset size:

4.07 GB

Imageset DOI:

10.6019/EMPIAR-10773

Experimental metadata:

Download xml json

Contains:

reconstructed volumes

Image Sets

1. Tomograms of rod outer segments in wild type mice acquired conventionally with defocus

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(928, 928)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(10.48, 10.48)

Details:

4x binned and dose filtered tomograms with dimensions 928x928x464. Tilt series were acquired conventionally with defocus. Projection images were recorded between -50° and 50° with an increment of 2°. The 310 frames are distributed over all projections. The total exposure per tilt-series was ~ 100e-/A^2.

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Citations

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Burton Smith RN, Murata K. (2026)

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AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

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prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)