EMPIAR-10736 - Cryo-EM of the human insulin receptor ectodomain in complex with an insulin analog with truncated B chain and enlongated A chain [multiple data sets in MRC format]

EMPIAR-10736

Cryo-EM of the human insulin receptor ectodomain in complex with an insulin analog with truncated B chain and enlongated A chain [multiple data sets in MRC format]

Publication:

Symmetric and asymmetric receptor conformation continuum induced by a new insulin

Forbes BE, Safavi-Hemami H, Hill CP, Chou DH

Nature chemical biology 18 (2022) 511-519

PMID: 35289328

DOI: 10.1038/s41589-022-00981-0

Related EMDB entry:

EMD-23950 EMD-23951 EMD-23949

Deposited:

2021-05-12

Released:

2022-03-14

Last modified:

2022-09-15

Imageset size:

2.20 TB

Imageset DOI:

10.6019/EMPIAR-10736

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

The following three files were found corrupted and removed.

- movies-dose-60/FoilHole_22055695_Data_22053810_22053812_20191121_2111-86724.mrc
- movies-dose-60/FoilHole_22055696_Data_22053772_22053774_20191121_2113-86730.mrc
- movies-dose-60/FoilHole_22055696_Data_22053778_22053780_20191121_2113-86731.mrc

Original size:   12,569,361,786 kilobytes
Compressed size:  2,357,786,229 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs (40 e-/A2 dose)

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

7221

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.365, 1.365)

Details:

Raw, uncurated cryo-EM movies. Not gain corrected. Use gain image matching pattern *-gain-ref.mrc

Files:

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. unaligned micrographs (60 e-/A2 dose)

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4078

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.365, 1.365)

Details:

Raw, uncurated cryo-EM movies. Not gain corrected. Use gain image matching pattern *-gain-ref.mrc

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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