EMPIAR-10708 - Apoferritin, TMV and T20S proteasome in nanofluidic channels [multiple data sets in TIFF format]

EMPIAR-10708

Apoferritin, TMV and T20S proteasome in nanofluidic channels [multiple data sets in TIFF format]

Publication:

Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes

Huber ST, Sarajlic E, Huijink R, Weis F, Evers WH, Jakobi AJ

eLife 11 (2022)

PMID: 35060902

DOI: 10.7554/elife.72629

Related EMDB entry:

EMD-12901 EMD-12915 EMD-12903

Deposited:

2021-05-07

Released:

2021-10-19

Last modified:

2022-02-28

Imageset size:

331.05 GB

Imageset DOI:

10.6019/EMPIAR-10708

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of pyrococcus furiosus apoferritin in silicon nitride nanochannels

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

948

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.8127, 0.8127)

Details:

Movies are not gain-corrected
gain reference is in data/ApoFtn/CountRef_map_0044_001.mrc
needs to be flipped in y direction
defect file is in data/ApoFtn/defects_map_0044_001.txt
3.4 mg/mL concentration of sample
63 e-/A**2 total exposure

Files:

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2. Multi-frame movies of T20S proteasome in silicon nitride nanochannels

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

121

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.288, 1.288)

Details:

Gain-corrected compressed tif movies
1.4 mg/mL concentration of sample
59.7 e-/A**2 total exposure

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Multi-frame movies of TMV in silicon nitride nanochannels

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.288, 1.288)

Details:

Gain-corrected compressed tif movies
1.1 mg/mL concentration of sample
59.7 e-/A**2 total exposure

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

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