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EMPIAR-10604
Single particle cryo-EM dataset of Mus musculus mitochondrial complex I in the deactive state [1768 multi-frame micrographs composed of 30 frames each in MRCS format]
Publication:

Structural basis for a complex I mutation that blocks pathological ROS production

Yin Z, Burger N, Kula-Alwar D, Aksentijević D, Bridges HR, Prag H, Grba D, Viscomi C, James AJ, Mottahedin A, Kreig T, Murphy MP, Hirst J

Nature communications 12 (2021)

PMID: 33514727

Related EMDB entry:
EMD-11810 EMD-4356
Deposited:
2020-12-07
Released:
2021-02-12
Last modified:
2021-02-12
Imageset size:
2.75 TB
Imageset DOI:
10.6019/EMPIAR-10604
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
Image Sets
1. Single particle cryo-EM dataset of Mus musculus mitochondrial complex I in the deactive state
Category:
micrographs - multiframe
Image format:
MRCS
No. of images or tilt series:
1768
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.35, 1.35)
Details:
Micrographs were imaged using a Gatan K2 summit camera in counting mode and GIF Quantum energy filter (20 eV slit width) on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. 15 s exposures captured in 30 frames and a total dose of 50 electrons Å-2
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Citations
AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.
Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)
A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.
Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)
Helical reconstruction of amyloids in cryoSPARC.
Schaefer JH, O'Neill RT, Donnelly JP, Powers ET, Kelly JW, Lander GC. (2026)
An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.
Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)
CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.
Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)
prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.
He L, Bartesaghi A. (2026)
CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.
Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)
Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.
Kong L, Zottig X, Elferich J, Grigorieff N. (2026)
CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.
Gyawali R, Dhakal A, Wang L, Cheng J. (2026)
Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.
Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)
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