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Electron Microscopy Public Image Archive

EMPIAR-10560
Nudaurelia capensis omega virus capsid: virus-like particles expressed in Nicotiana benthamiana [multiple data sets in MRC format]
Publication:

Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus

Castells-Graells R, Ribeiro JRS, Domitrovic T, Hesketh EL, Scarff CA, Johnson JE, Ranson NA, Lawson DM, Lomonossoff GP

Communications biology 4 (2021)

PMID: 34031522

Related EMDB entry:
EMD-11830
Deposited:
2020-11-17
Released:
2022-02-04
Last modified:
2022-02-04
Imageset size:
3.39 TB
Imageset DOI:
10.6019/EMPIAR-10560
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (Images-Disc1/*/FoilHole*Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   5,390,076,648 kilobytes
Compressed size: 3,432,762,789  kilobytes
Contains:
  • micrographs - multiframe
  • micrographs - single frame
Image Sets
1. unaligned movies
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
2788
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.097, 1.097)
Details:
Micrographs are unaligned movies comprised of 59 frames each

data/motion_corrected_data/shift_eps_files - EPS files showing motion correction trajectories for each movie in the dataset.

data/motion_corrected_data/star_files - Star files from motion correction job.
Files:
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2. aligned and dose-weighted micrographs
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2788
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.0651, 1.0651)
Details:
Micrographs are averages of aligned and dose-weighted movies comprised of 59 frames

data/motion_corrected_data/shift_eps_files - EPS files showing motion correction trajectories for each movie in the dataset.

data/motion_corrected_data/star_files - Star files from motion correction job.
Files:
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Citations
Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.
Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Outer membrane protein assembly mediated by BAM-SurA complexes.
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)
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