EMPIAR-10560 - Nudaurelia capensis omega virus capsid: virus-like particles expressed in Nicotiana benthamiana [multiple data sets in MRC format]

EMPIAR-10560

Nudaurelia capensis omega virus capsid: virus-like particles expressed in Nicotiana benthamiana [multiple data sets in MRC format]

Publication:

Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus

Castells-Graells R, Ribeiro JRS, Domitrovic T, Hesketh EL, Scarff CA, Johnson JE, Ranson NA, Lawson DM, Lomonossoff GP

Communications biology 4 (2021)

PMID: 34031522

DOI: 10.1038/s42003-021-02134-w

Related EMDB entry:

EMD-11830

Deposited:

2020-11-17

Released:

2022-02-04

Last modified:

2022-02-04

Imageset size:

3.39 TB

Imageset DOI:

10.6019/EMPIAR-10560

Experimental metadata:

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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (Images-Disc1/*/FoilHole*Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   5,390,076,648 kilobytes
Compressed size: 3,432,762,789  kilobytes

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. unaligned movies

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2788

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.097, 1.097)

Details:

Micrographs are unaligned movies comprised of 59 frames each

data/motion_corrected_data/shift_eps_files - EPS files showing motion correction trajectories for each movie in the dataset.

data/motion_corrected_data/star_files - Star files from motion correction job.

Files:

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2. aligned and dose-weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

2788

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.0651, 1.0651)

Details:

Micrographs are averages of aligned and dose-weighted movies comprised of 59 frames

data/motion_corrected_data/shift_eps_files - EPS files showing motion correction trajectories for each movie in the dataset.

data/motion_corrected_data/star_files - Star files from motion correction job.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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