EMPIAR-10461 - SBF SEM images of a Zebrafish hindbrain macrophage containing 2 Toxoplasma gondii tachizoites [multiple data sets in DM4 format]

EMPIAR-10461

SBF SEM images of a Zebrafish hindbrain macrophage containing 2 Toxoplasma gondii tachizoites [multiple data sets in DM4 format]

Publication:

The zebrafish as a novel model for the in vivo study of Toxoplasma gondii replication and interaction with macrophages

Yoshida N, Domart MC, Peddie CJ, Yakimovich A, Mazon-Moya MJ, Hawkins TA, Collinson L, Mercer J, Frickel EM, Mostowy S

Disease models & mechanisms 13 (2020)

PMID: 32461265

DOI: 10.1242/dmm.043091

Related EMDB entry:

Deposited:

2020-07-03

Released:

2021-01-22

Last modified:

2022-02-08

Imageset size:

16.49 GB

Imageset DOI:

10.6019/EMPIAR-10461

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Survey SBF SEM image showing the position of the imaged ROIs at the start of the run

Category:

micrographs - single frame

Image format:

DM4

No. of images or tilt series:

Image size:

(6000, 5000)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(472.5, 472.5)

Details:

Survey image showing the position of the ROIs imaged (ROI00 and ROI01) if opened in Digital Micrograph

Files:

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2. Low magnification raw SBF SEM overview images (ROI00) of a Toxoplasma Gondii tachizoite in a Zebrafish hindbrain macrophage

Category:

micrographs - single frame

Image format:

DM4

No. of images or tilt series:

133

Image size:

(6000, 5000)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(332.1, 332.1)

Details:

Low magnification overview images (ROI00) linked to higher magnification images in ROI01. Imaged at 2kV, 2us dwell, high vacuum with focal charge compensation on at 50%

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. High magnification raw SBF SEM images (ROI01) of a Toxoplasma Gondii tachizoite in a Zebrafish hindbrain macrophage

Category:

micrographs - single frame

Image format:

DM4

No. of images or tilt series:

132

Image size:

(6000, 6000)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(60.0, 60.0)

Details:

High magnification images (ROI01) linked to lower magnification images in ROI00. Imaged at 2kV, 2us dwell, high vacuum with focal charge compensation on at 50%

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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