EMPIAR-10451 - Cryo electron tomography after FIB-milling of Planctomycetes species Tuwongella immobilis (33k magnification) [multiple data sets in MRC format]

EMPIAR-10451

Cryo electron tomography after FIB-milling of Planctomycetes species Tuwongella immobilis (33k magnification) [multiple data sets in MRC format]

Publication:

Evolutionary Remodeling of the Cell Envelope in Bacteria of the Planctomycetes Phylum

Mahajan M, Seeger C, Yee B, Andersson SGE

Genome biology and evolution 12 (2020) 1528-1548

PMID: 32761170

DOI: 10.1093/gbe/evaa159

Related EMDB entry:

EMD-11124

Deposited:

2020-06-23

Released:

2020-12-11

Last modified:

2020-12-11

Imageset size:

15.46 GB

Imageset DOI:

10.6019/EMPIAR-10451

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
tilt series

Image Sets

1. Single micrographs from cryo-ET tilt series (uncorrected) of Tuwongella immobilis after FIB-milling (33k magnification)

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(4.291, 4.291)

Details:

Uncorrected micrographs from cryo-ET tilt series from Tuwongella immobilis obtained after FIB-milling. Total electron dose: 95e-/Å^2; 61 tilts; -60°/+60°, 2° increment, start at +20°; defocus: -4µm

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2. Single micrographs from cryo-ET tilt series (motion-corrected) of Tuwongella immobilis after FIB-milling (33k magnification)

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(4.291, 4.291)

Details:

Motion-corrected micrographs from cryo-ET tilt series from Tuwongella immobilis obtained after FIB-milling. Total electron dose: 95e-/Å^2; 61 tilts; -60°/+60°, 2° increment, start at +20°; defocus: -4µm

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Image stack (motion-corrected) from cryo-ET tilt series of Tuwongella immobilis after FIB-milling (33k magnification)

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(4.291, 4.291)

Details:

Image stack (motion corrected) from cryo-ET tilt series from Tuwongella immobilis obtained after FIB-milling. Total electron dose: 95e-/Å^2; 61 tilts; -60°/+60°, 2° increment, start at +20°; defocus: -4µm

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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