EMPIAR-10451
Cryo electron tomography after FIB-milling of Planctomycetes species Tuwongella immobilis (33k magnification) [multiple data sets in MRC format]
Publication:

Evolutionary Remodeling of the Cell Envelope in Bacteria of the Planctomycetes Phylum

Mahajan M, Seeger C, Yee B, Andersson SGE

Genome biology and evolution 12 (2020) 1528-1548

PMID: 32761170

Related EMDB entry:
Deposited:
2020-06-23
Released:
2020-12-11
Last modified:
2020-12-11
Imageset size:
15.46 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
  • tilt series
1. Single micrographs from cryo-ET tilt series (uncorrected) of Tuwongella immobilis after FIB-milling (33k magnification)
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
61
Image size:
(3710, 3838)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(4.291, 4.291)
Details:
Uncorrected micrographs from cryo-ET tilt series from Tuwongella immobilis obtained after FIB-milling. Total electron dose: 95e-/Å^2; 61 tilts; -60°/+60°, 2° increment, start at +20°; defocus: -4µm
Files:
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2. Single micrographs from cryo-ET tilt series (motion-corrected) of Tuwongella immobilis after FIB-milling (33k magnification)
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
61
Image size:
(3710, 3838)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(4.291, 4.291)
Details:
Motion-corrected micrographs from cryo-ET tilt series from Tuwongella immobilis obtained after FIB-milling. Total electron dose: 95e-/Å^2; 61 tilts; -60°/+60°, 2° increment, start at +20°; defocus: -4µm
Files:
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3. Image stack (motion-corrected) from cryo-ET tilt series of Tuwongella immobilis after FIB-milling (33k magnification)
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
61
Image size:
(3710, 3838)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(4.291, 4.291)
Details:
Image stack (motion corrected) from cryo-ET tilt series from Tuwongella immobilis obtained after FIB-milling. Total electron dose: 95e-/Å^2; 61 tilts; -60°/+60°, 2° increment, start at +20°; defocus: -4µm
Files:
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Files:
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