EMPIAR-10317 - Cryo-EM dataset, containing multiple conformations of a heterodimeric ABC exporter TmrAB (EQ-mutant), incubated with a mixture of ATP and ADP [multiple data sets in MRC and MRCS formats]

EMPIAR-10317

Cryo-EM dataset, containing multiple conformations of a heterodimeric ABC exporter TmrAB (EQ-mutant), incubated with a mixture of ATP and ADP [multiple data sets in MRC and MRCS formats]

Publication:

Conformation space of a heterodimeric ABC exporter under turnover conditions

Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Brüchert S, Kuhn BT, Geertsma ER, Hummer G, Tampé R, Moeller A

Nature 571 (2019) 580-583

PMID: 31316210

DOI: 10.1038/s41586-019-1391-0

Related EMDB entry:

EMD-4775 EMD-4776

Deposited:

2019-08-26

Released:

2019-11-06

Last modified:

2019-11-19

Imageset size:

7.13 TB

Imageset DOI:

10.6019/EMPIAR-10317

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - multiframe - unprocessed

Image Sets

1. Unaligned multi-frame micrographs of a heterodimeric ABC exporter TmrAB (EQ-mutant) in multiple ATP/ADP bound conformations

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3263

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.077, 1.077)

Details:

Unaligned, gain-corrected, 40-frame movies, recorded automatically with EPU, using K2 camera in counting mode on a Titan Krios microscope operated at 300 kV at a nominal magnification of 130 000, corresponding to a pixel size of 1.077 Å. The total electron dose per micrograph - approximately 62 electrons per squared Angstrom.

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2. TmrAB(EQ) particles in multiple conformations after one round of cleaning in 3D

Category:

picked particles - multiframe - unprocessed

Image format:

MRCS

No. of images or tilt series:

1547017

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.077, 1.077)

Details:

1.5 mln particle stack (box size - 256 pixels, 1.077 Å/pixel) of TmrAB(EQ) in multiple conformations after a single round of cleaning in 3D (particles picked and extracted in Relion-3).

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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