EMPIAR-10317 - Cryo-EM dataset, containing multiple conformations of a heterodimeric ABC exporter TmrAB (EQ-mutant), incubated with a mixture of ATP and ADP [multiple data sets in MRC and MRCS formats]

EMPIAR-10317

Cryo-EM dataset, containing multiple conformations of a heterodimeric ABC exporter TmrAB (EQ-mutant), incubated with a mixture of ATP and ADP [multiple data sets in MRC and MRCS formats]

Publication:

Conformation space of a heterodimeric ABC exporter under turnover conditions

Hofmann S, Januliene D, Mehdipour AR, Thomas C, Stefan E, Brüchert S, Kuhn BT, Geertsma ER, Hummer G, Tampé R, Moeller A

Nature 571 (2019) 580-583

PMID: 31316210

DOI: 10.1038/s41586-019-1391-0

Related EMDB entry:

EMD-4775 EMD-4776

Deposited:

2019-08-26

Released:

2019-11-06

Last modified:

2019-11-19

Imageset size:

7.13 TB

Imageset DOI:

10.6019/EMPIAR-10317

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - multiframe - unprocessed

Image Sets

1. Unaligned multi-frame micrographs of a heterodimeric ABC exporter TmrAB (EQ-mutant) in multiple ATP/ADP bound conformations

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3263

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.077, 1.077)

Details:

Unaligned, gain-corrected, 40-frame movies, recorded automatically with EPU, using K2 camera in counting mode on a Titan Krios microscope operated at 300 kV at a nominal magnification of 130 000, corresponding to a pixel size of 1.077 Å. The total electron dose per micrograph - approximately 62 electrons per squared Angstrom.

Files:

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2. TmrAB(EQ) particles in multiple conformations after one round of cleaning in 3D

Category:

picked particles - multiframe - unprocessed

Image format:

MRCS

No. of images or tilt series:

1547017

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.077, 1.077)

Details:

1.5 mln particle stack (box size - 256 pixels, 1.077 Å/pixel) of TmrAB(EQ) in multiple conformations after a single round of cleaning in 3D (particles picked and extracted in Relion-3).

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

See all citations

Protein Data Bank Japan News

[2026-06-10]

333件のPDBエントリーが新たに公開されました。（2026-06-10付）