EMPIAR-10299 - PolyA polymerase module of the cleavage and polyadenylation factor (CPF) from Saccharomyces cerevisiae [multiple data sets in MRCS format]

EMPIAR-10299

PolyA polymerase module of the cleavage and polyadenylation factor (CPF) from Saccharomyces cerevisiae [multiple data sets in MRCS format]

Publication:

Architecture of eukaryotic mRNA 3′-end processing machinery

Casañal A, Kumar A, Hill CH, Easter AD, Emsley P, Degliesposti G, Gordiyenko Y, Santhanam B, Wolf J, Wiederhold K, Dornan GL, Skehel M, Robinson CV, Passmore LA

Science (New York, N.Y.) 358 (2017) 1056-1059

PMID: 29074584

DOI: 10.1126/science.aao6535

Related EMDB entry:

EMD-3908

Deposited:

2019-07-24

Released:

2019-10-14

Last modified:

2019-10-14

Imageset size:

15.37 TB

Imageset DOI:

10.6019/EMPIAR-10299

Experimental metadata:

Download xml json

Contains:

picked particles - multiframe - processed
micrographs - multiframe

Image Sets

1. Polished shiny particles

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

459867

Image size:

(128, 128)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

This folder contains particles polished in RELION 3.1. The nominal pixel size is 1.40 A/px except for MRCS_Diamond folder, which is 1.36 A/px. The particles were pre-multiplied by CTF before cropping to counter the effect of signal delocalisation by CTF. The STAR file is not compatible with earlier versions of RELION.

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2. Unaligned super-resolution movies

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

3952

Image size:

(7676, 7420)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

This dataset was collected over five sessions.

Common parameters:
300 kV
Cs 2.7 mm

Nominal pixel sizes:
MRCS_Diamond: 0.68 Å / super-resolution pixel
Others: 0.70 Å / super-resolution pixel

Nominal dose rate:
MRCS_GAIN_1608: 2.24 e/A2/frame
MRCS_GAIN_1609: 2.0 e/A2/frame
MRCS_GAIN_1611: 2.23 e/A2/frame
MRCS_GO_161202: 1.8 e/A2/frame
MRCS_Diamond: 2.24 e/A2/frame

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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