EMPIAR-10299 - PolyA polymerase module of the cleavage and polyadenylation factor (CPF) from Saccharomyces cerevisiae [multiple data sets in MRCS format]

EMPIAR-10299

PolyA polymerase module of the cleavage and polyadenylation factor (CPF) from Saccharomyces cerevisiae [multiple data sets in MRCS format]

Publication:

Architecture of eukaryotic mRNA 3′-end processing machinery

Casañal A, Kumar A, Hill CH, Easter AD, Emsley P, Degliesposti G, Gordiyenko Y, Santhanam B, Wolf J, Wiederhold K, Dornan GL, Skehel M, Robinson CV, Passmore LA

Science (New York, N.Y.) 358 (2017) 1056-1059

PMID: 29074584

DOI: 10.1126/science.aao6535

Related EMDB entry:

EMD-3908

Deposited:

2019-07-24

Released:

2019-10-14

Last modified:

2019-10-14

Imageset size:

15.37 TB

Imageset DOI:

10.6019/EMPIAR-10299

Experimental metadata:

Download xml json

Contains:

picked particles - multiframe - processed
micrographs - multiframe

Image Sets

1. Polished shiny particles

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

459867

Image size:

(128, 128)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

This folder contains particles polished in RELION 3.1. The nominal pixel size is 1.40 A/px except for MRCS_Diamond folder, which is 1.36 A/px. The particles were pre-multiplied by CTF before cropping to counter the effect of signal delocalisation by CTF. The STAR file is not compatible with earlier versions of RELION.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Unaligned super-resolution movies

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

3952

Image size:

(7676, 7420)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

This dataset was collected over five sessions.

Common parameters:
300 kV
Cs 2.7 mm

Nominal pixel sizes:
MRCS_Diamond: 0.68 Å / super-resolution pixel
Others: 0.70 Å / super-resolution pixel

Nominal dose rate:
MRCS_GAIN_1608: 2.24 e/A2/frame
MRCS_GAIN_1609: 2.0 e/A2/frame
MRCS_GAIN_1611: 2.23 e/A2/frame
MRCS_GO_161202: 1.8 e/A2/frame
MRCS_Diamond: 2.24 e/A2/frame

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

See all citations

Protein Data Bank Japan News

[2024-04-24]

354 new PDB entries have been released on 2024-04-24.

[2024-04-09]

[wwPDB] CASP16 Call for Targets

[2024-04-02]

Announcement of the PDBj Newsletter Vol.24 release

[2024-03-21]

[wwPDB] Paper Published on NMR Restraint Validation

[2024-03-13]

[wwPDB] Paper Published on CryoEM Archiving and Validation Recommendations

[2024-03-06]

[wwPDB] Poster Prize Awarded at The Biophysical Society Meeting

[2024-02-15]

Notice of Service Suspension (ASH, DASH, SeSAW, MAFFTash, Spanner, SFAS)

[2024-02-13]

[wwPDB] Biocurator Milestone: >10,000 Depositions Processed

[2024-02-06]

[wwPDB] Preprint Published on NMR Restraint Validation

[2024-02-05]

[wwPDB] Preprint Published on CryoEM Archiving and Validation Recommendations

See all news