micrographs - multiframe

MRC

2384

(3838, 3710)

32 BIT FLOAT

(1.12, 1.12)

Unaligned, gain-corrected, 20-frame movies from K2 in counting mode. Data collected with EPU using a Titan Krios operated at 300 kV. Each micrograph accumulate 47 electrons per square Angstrom over 20 frames (12 s). Nominal pixel size is 1.12 Å/pixel, this is uncalibrated. This is the full dataset, so several "bad" micrographs will be present - during data processing, we discarded 89 micrographs that had ice contamination or power spectra.

The attached star file (mergeddata_polished_refined_data.star) is the final refinement star file for the 3.3 Å reconstruction deposited as EMD-10140, after polishing in RELION 2.0 and per-particle CTF refinement. The micrograph paths have been altered to correspond to the directory structure of this deposition, so the X and Y coordinates might be useful, but this refinement was performed on a small subset of particles in one particular state so many more particles will be found in each micrograph.

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micrographs - multiframe

MRC

1614

(3838, 3710)

32 BIT FLOAT

(0.88, 0.88)

Unaligned, gain-corrected, 35-frame movies from K2. 173 are in super-resolution mode, 1441 are in counting mode. Data collected with EPU using a Titan Krios operated at 300 kV. Each micrograph accumulate 45 electrons per square Angstrom over 35 frames (7 s). Pixel size is 0.88 Å/pixel, calibrated by correlating the calculated map to the map from Dataset 1 using a reference pixel size of 1.12 Å. This is the full dataset, so several "bad" micrographs will be present. The super-resolution micrographs are not helpfully separated from the counting micrographs, I suggest during processing to perform a command like "find data/Movies_23D/ -size +3G" to separate the super-resolution micrographs.

The attached star file (mergeddata_polished_refined_data.star) is the final refinement star file for the 3.3 Å reconstruction deposited as EMD-10140, after polishing in RELION 2.0 and per-particle CTF refinement. The micrograph paths have been altered to correspond to the directory structure of this deposition, so the X and Y coordinates might be useful, but this refinement was performed on a small subset of particles in one particular state so many more particles will be found in each micrograph. The X and Y coordinates for the super-resolution micrographs refer to 2x binned micrographs.

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