Unaligned, gain-corrected, 20-frame movies from K2 in counting mode. Data collected with EPU using a Titan Krios operated at 300 kV. Each micrograph accumulate 47 electrons per square Angstrom over 20 frames (12 s). Nominal pixel size is 1.12 Å/pixel, this is uncalibrated. This is the full dataset, so several "bad" micrographs will be present - during data processing, we discarded 89 micrographs that had ice contamination or power spectra.
The attached star file (mergeddata_polished_refined_data.star) is the final refinement star file for the 3.3 Å reconstruction deposited as EMD-10140, after polishing in RELION 2.0 and per-particle CTF refinement. The micrograph paths have been altered to correspond to the directory structure of this deposition, so the X and Y coordinates might be useful, but this refinement was performed on a small subset of particles in one particular state so many more particles will be found in each micrograph.
Files:
Available download options:
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
Unaligned, gain-corrected, 35-frame movies from K2. 173 are in super-resolution mode, 1441 are in counting mode. Data collected with EPU using a Titan Krios operated at 300 kV. Each micrograph accumulate 45 electrons per square Angstrom over 35 frames (7 s). Pixel size is 0.88 Å/pixel, calibrated by correlating the calculated map to the map from Dataset 1 using a reference pixel size of 1.12 Å. This is the full dataset, so several "bad" micrographs will be present. The super-resolution micrographs are not helpfully separated from the counting micrographs, I suggest during processing to perform a command like "find data/Movies_23D/ -size +3G" to separate the super-resolution micrographs.
The attached star file (mergeddata_polished_refined_data.star) is the final refinement star file for the 3.3 Å reconstruction deposited as EMD-10140, after polishing in RELION 2.0 and per-particle CTF refinement. The micrograph paths have been altered to correspond to the directory structure of this deposition, so the X and Y coordinates might be useful, but this refinement was performed on a small subset of particles in one particular state so many more particles will be found in each micrograph. The X and Y coordinates for the super-resolution micrographs refer to 2x binned micrographs.
Files:
Available download options:
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
