EMPIAR-10180 - Structure of a pre-catalytic spliceosome [stack of 327490 particles in MRCS format]

EMPIAR-10180

Structure of a pre-catalytic spliceosome [stack of 327490 particles in MRCS format]

Publication:

Structure of a pre-catalytic spliceosome

Plaschka C, Lin PC, Nagai K

Nature 546 (2017) 617-621

PMID: 28530653

DOI: 10.1038/nature22799

Related EMDB entry:

EMD-3683 EMD-3686 EMD-3687 EMD-3685 EMD-3688 EMD-3682 EMD-3684

Deposited:

2018-03-27

Released:

2018-05-30

Last modified:

2018-08-30

Imageset size:

126.48 GB

Imageset DOI:

10.6019/EMPIAR-10180

Experimental metadata:

Download xml json

Contains:

picked particles - single frame - processed

Image Sets

1. Polished parcitles

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

327490

Image size:

(320, 320)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.699, 1.699)

Details:

Shiny particles after particle polishing used for the Nature paper were further down-sampled to 1.699 Å/px and windowed to 320 px. These particles were used in the Multibody paper.

The particle STAR file after consensus refinement, masks and initial references for four bodies and a body STAR file are also included. Readers can reproduce the multibody refinement in the paper using these inputs.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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