EMPIAR-10116 - Raw 2d tomographic tilt series of a dividing cell [65 tilt series in Other format]

EMPIAR-10116

Raw 2d tomographic tilt series of a dividing cell [65 tilt series in Other format]

Publication:

Postmitotic nuclear pore assembly proceeds by radial dilation of small membrane openings

Otsuka S, Steyer AM, Schorb M, Hériché JK, Hossain MJ, Sethi S, Kueblbeck M, Schwab Y, Beck M, Ellenberg J

Nature structural & molecular biology 25 (2017) 21-28

PMID: 29323269

DOI: 10.1038/s41594-017-0001-9

Related EMDB entry:

EMD-3820

Deposited:

2017-06-27

Released:

2018-02-07

Last modified:

2018-02-07

Imageset size:

237.84 GB

Imageset DOI:

10.6019/EMPIAR-10116

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Raw 2D tilt series for all the electron tomograms

Category:

tilt series

Image format:

Other

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(7.5, 7.5)

Details:

HeLa cells were instantly frozen using a high-pressure freezing machine (HPM 010; ABRA Fluid AG, Widnau, Switzerland) and freeze-substituted into Lowicryl HM20 resin (Polysciences Inc., Warrington, PA). As fiducial markers, 15 nm of gold-conjugated Protein A (CMC university Medical Center Utrecht, Utrecht, Netherlands) was absorbed on both sides of 300 nm sections. Sections were post-stained with 2% UA and lead citrate. Single or dual axis tilt series were acquired with a TECNAI TF30 trans-mission EM (TEM; 300 kV; FEI, Hillsboro, OR) equipped with a 4k x 4k Eagle camera (FEI) by using the Serial EM software. Images were recorded over a -60° to 60° tilt range with an angular increment 1°.

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Citations

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