The pixel size in xy was set to be 5 nm and the FIB-milling was done every 8 nm. The acquired raw FIB-SEM images were aligned using TrackEM2 (Cardona et al., PloS one, e38011, 2012) in ImageJ (http://rsbweb.nih.gov/ij/). After alignment, the stack was cropped, gray levels were inverted and the images were smoothened with a mean filter (kernel size: 15 × 15 nm). We used the left half of the cell for segmentation.
Files:
Available download options:
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.