EMPIAR-14008
Focused ion beam-scanning electron microscopy volume of the C. elegans -1 oocyte and surrounding tissue in zarr2 format [5985 micrographs in BIG DATA VIEWER HDF5 format]
Publication:

Organelle Volume Facilitates Spindle Positioning During C. elegans Female Meiosis

Lazureanu DG, Krueger EW, Qiu W, Kopley-Smith AA, Smith CS, Aquino AP, Lele A, Voloshchuk N, Cortes DB, Park M, Uryo H, Silva M, Trautman E, Innerberger M, Preibisch S, Vaxenburg R, Stark A, Bleck CKE, Reilly M, Wang MC, Shroff H, McNally FJ

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Related EMDB entry:
Deposited:
2026-09-01
Released:
2026-10-21
Last modified:
2026-10-22
Imageset size:
591.09 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. Focused ion beam-scanning electron microscopy volume of the C. elegans -1 oocyte and surrounding tissue in zarr2 format
Category:
micrographs - single frame
Image format:
BIG DATA VIEWER HDF5
No. of images or tilt series:
5985
Image size:
(10526, 14451)
Pixel type:
BIT
Pixel spacing:
(60.0, 60.0)
Details:
The volume was acquired at 6 nm isotropic resolution using a customized Zeiss FIB-SEM system.
Landing energy: 0.9 keV
Beam current: 2.0 nA
InLens detector
Working distance: 3.1 mm
Scan rate: 0.5 MHz
FIB milling: 30 kV gallium at 18 nA

The raw data was registered and aligned using the Render software pipeline (https://github.com/saalfeldlab/render) and exported as a multiscale zarr volume.
Files:
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