EMPIAR-13905
Cryo-EM datasets of human Argonaute2 pre-RISC and mature RISC-target, PDB 9YM9, 9YMA, 9YMB, 9YMC, 9YMD, and 10BE [multiple data sets in TIFF and EER formats]
Publication:

Structural basis for RISC assembly of human Argonaute2

Zhang H, Adhav VA, Kehling AC, Savidge A, Shen Z, Fu TM, Nakanishi K

Molecular cell 86 (2026) 2088-210500000000.0

PMID: 42190652

Deposited:
2026-09-01
Released:
2026-10-07
Last modified:
2026-10-07
Imageset size:
11.85 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Human AGO2 in complex with miR-20a duplex at 4 ºC_pre-RISC dataset 1
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
6164
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
#1 Raw images of pre-RISC at 4 ºC using a 300-kV Titan Krios microscope equipped with a K3 direct electron detector in super-resolution mode, with magnification of 81,000x, a pixel size of 0.426 A ̊ , a defocus range from -0.6 to -1.6 μm, and a total dose of 50 e- A ̊-2 over 50 fractions.
Files:
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2. Human AGO2 in complex with miR-20a duplex at 4 ºC_pre-RISC dataset 2
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3927
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
#2 Raw images of pre-RISC at 4 ºC using a 300-kV Titan Krios microscope equipped with a K3 direct electron detector in super-resolution mode, with magnification of 81,000x, a pixel size of 0.426 A ̊ , a defocus range from -0.6 to -1.6 μm, and a total dose of 50 e- A ̊-2 over 50 fractions.
Files:
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3. Human AGO2 in complex with miR-20a duplex at 21 ºC_pre-RISC dataset 3
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
11296
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Raw images of pre-RISC at 21 ºC using a 300-kV Titan Krios microscope equipped with a K3 camera in counting mode, with magnification of 105,000x, a pixel size of 0.835 A ̊ , a defocus range from -0.8 to -2.4 μm, and a total dose of 50 e- A ̊ -2 over 40 fractions.
Files:
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4. Human AGO2 in complex with 23-nt miR-20a and an 18-nt target with 2 mismatches
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
8149
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(None, None)
Details:
Raw images of AGO2 in complex with 23-nt miR-20a and an 18-nt target with 2 mismatches using a Titan Krios microscope equipped with a Gatan K3 camera in counting mode, with magnification of 105,000 x, a pixel size of 0.7296 A ̊ , a defocus range from -0.8 to -2.2 μm, and a total dose of 50 e- A ̊ -2 over 50 fractions.
Files:
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