CryoEM movies of Mo-nitrogenase and FeSII from Azotobacter vinelandii in presence of sodium dithionite frozen under Al's oil [multiple data sets in EER and MRC formats]
Publication:
Structural basis for the conformational protection of nitrogenase from O2
Unaligned multi-frame micrographs of Mo-nitrogenase and FeSII from Azotobacter vinelandii in presence of sodium dithionite frozen under Al's oil collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.
Aligned micrographs of the catalytic subunit of of Mo-nitrogenase and FeSII from Azotobacter vinelandii in presence of sodium dithionite frozen under Al's oil collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.
Gain file required for motion correction of unaligned micrographs of the catalytic subunit of Mo-nitrogenase and FeSII from Azotobacter vinelandii in presence of sodium dithionite frozen under Al's oil