EMPIAR-13774
CryoEM movies of Mo-nitrogenase and FeSII from Azotobacter vinelandii in presence of sodium dithionite frozen under Al's oil [multiple data sets in EER and MRC formats]
Publication:

Structural basis for the conformational protection of nitrogenase from O2

Narehood SM, Cook BD, Srisantitham S, Eng VH, Shiau AA, McGuire KL, Britt RD, Herzik MA, Tezcan FA

Nature 637 (2025) 991-997

PMID: 39779844

Related EMDB entry:
Deposited:
2026-06-09
Released:
2026-11-12
Last modified:
2026-11-12
Imageset size:
796.80 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
  • micrographs - single frame
1. Unaligned multi-frame micrographs of Mo-nitrogenase and FeSII from Azotobacter vinelandii in presence of sodium dithionite frozen under Al's oil
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
1580
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.735, 0.735)
Details:
Unaligned multi-frame micrographs of Mo-nitrogenase and FeSII from Azotobacter vinelandii in presence of sodium dithionite frozen under Al's oil collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.
Files:
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2. Aligned micrographs of Mo-nitrogenase and FeSII from Azotobacter vinelandii in presence of sodium dithionite frozen under Al's oil
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1580
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(None, None)
Details:
Aligned micrographs of the catalytic subunit of of Mo-nitrogenase and FeSII from Azotobacter vinelandii in presence of sodium dithionite frozen under Al's oil collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.
Files:
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3. Gain file for motion correction of unaligned micrographs of Mo-nitrogenase and FeSII from Azotobacter vinelandii in presence of sodium dithionite frozen under Al's oil
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(None, None)
Details:
Gain file required for motion correction of unaligned micrographs of the catalytic subunit of Mo-nitrogenase and FeSII from Azotobacter vinelandii in presence of sodium dithionite frozen under Al's oil
Files:
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