EMPIAR-13728 - JMV449-NTSR1-Gi in 8 seconds following the GTP addition [12138 multi-frame micrographs composed of 48 frames each in TIFF format]

EMPIAR-13728

JMV449-NTSR1-Gi in 8 seconds following the GTP addition [12138 multi-frame micrographs composed of 48 frames each in TIFF format]

Publication:

The dynamic basis of G-protein recognition and activation by a GPCR

Kobayashi K, Kawakami K, Matsui TE, Yokoi S, Fukuda M, Narita TJ, Arai H, Tambo M, Sumikama T, Tatsumi M, Yamashita K, Koyanagi J, Kugawa M, Ikeda H, Sumino A, Mitsutake A, Kobilka BK, Inoue A, Kato HE

Nature 652 (2026) 812-821

PMID: 41813902

DOI: 10.1038/s41586-026-10228-w

Related EMDB entry:

EMD-64918 EMD-64917 EMD-64919

Deposited:

2026-02-19

Released:

2026-10-02

Last modified:

2026-10-02

Imageset size:

2.73 TB

Imageset DOI:

10.6019/EMPIAR-13728

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. imageset_1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

12138

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Please ignore the pixel size in the header (1.00 Å/px); use 0.83 Å/px.
- Cs: 2.7mm
- HT: 300 kV
- Dose per frame: 1 electron/A^2/frame
- Please use "movie_gain.mrc" as a gain reference with flipping along Y for CryoSPARC

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CryoMV: Structure-Prior-Guided Modeling and Real-Particle Validation of Continuous Conformational Transitions in Cryo-EM

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Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

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