EMPIAR-13658
Cryo-EM Structures of Methylation-dependent Cas9 [multiple data sets in MRC format]
Publication:

Molecular basis for methylation-sensitive editing by Cas9

Roth MO, Shu Y, Zhao Y, Trasanidou D, Hoffman RD, Südfeld C, Bouzetos E, Trasanidis N, Zawrotny M, Gelasco MK, Medina ML, Das A, Rai J, Goswami HN, Wang B, van der Oost J, Li H

Nature 653 (2026) 1229-1239

PMID: 41986708

Deposited:
2026-06-11
Released:
2026-06-28
Last modified:
2026-06-28
Imageset size:
11.09 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. unaligned multi-frame micrographs of ThermoCas9 incubated with methylated DNA target with NNNNCCA PAM
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
6603
Image size:
(11520, 8184)
Pixel type:
BIT
Pixel spacing:
(0.415, 0.415)
Details:
This dataset was collected on K3 camera at 105k magnification using EPU, with a super-resolution pixel size of 0.415 and a total dose of 60 spreading over 60 frames. Gain file is also provided. Gain orientation in Relion: no rotation, Flip upside-down; in cryosparc: no rotation, no flip.
Files:
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2. unaligned multi-frame micrographs of ThermoCas9 incubated with non-methylated DNA target with NNNNCCA PAM
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
6080
Image size:
(11520, 8184)
Pixel type:
BIT
Pixel spacing:
(0.415, 0.415)
Details:
This dataset was collected on K3 camera at 105k magnification using EPU, with a super-resolution pixel size of 0.415 and a total dose of 60 spreading over 61 frames. Gain file is also provided. Gain orientation in Relion: no rotation, Flip upside-down; in cryosparc: no rotation, no flip.
Files:
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3. unaligned multi-frame micrographs of ThermoCas9 incubated with non-methylated DNA target with NNNNCGA PAM
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
12596
Image size:
(11520, 8184)
Pixel type:
BIT
Pixel spacing:
(0.415, 0.415)
Details:
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Files:
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