EMPIAR-13600 - Single particle cryo-EM of Staphylococcus aureus YhaM D193A hexamers in complex with double-stranded substrate RNA derived from hpf mRNA [15724 multi-frame micrographs composed of 60 frames each in EER format]

EMPIAR-13600

Single particle cryo-EM of Staphylococcus aureus YhaM D193A hexamers in complex with double-stranded substrate RNA derived from hpf mRNA [15724 multi-frame micrographs composed of 60 frames each in EER format]

Publication:

Structural insights into RNA recognition by the Staphylococcus aureus exoribonuclease YhaM

Mattingly JM, Liponska A, Tanquary JR, Yap MNF, Dunham CM

Proceedings of the National Academy of Sciences (2026)

Related EMDB entry:

EMD-73296 EMD-73295

Deposited:

2026-05-21

Released:

2026-06-11

Last modified:

2026-06-11

Imageset size:

7.66 TB

Imageset DOI:

10.6019/EMPIAR-13600

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned EER format micrographs of S. aureus YhaM bound to hairpin RNA substrate derived from the hpf mRNA

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

15724

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.73, 0.73)

Details:

Micrographs in the "frames" directory are unaligned EER files collected with a pixel size of 0.73 A/px on a TFS Krios G4 (operating voltage of 300 kV) with an accumulated dose of 52.97 e-/A^2. Associated XML files in the "xml" directory contain EPU-format image shift data that can be used for optics group splitting for per-group CTF parameter refinement.

Files:

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Citations

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Protein Data Bank Japan News

[2026-06-10]

333件のPDBエントリーが新たに公開されました。（2026-06-10付）