EMPIAR-13472
Movies of alpha-hemolysin in the lipidic environment (Egg-PC/Chol with Hla) [3843 multi-frame micrographs composed of 20 frames each in MRC format]
Publication:

Structural insights into pre-pore intermediates of alpha-hemolysin in the lipidic environment

Chatterjee A, Roy A, Satheesh T, Das PP, Mondal B, Kishore P, Ganji M, Dutta S

Nature communications 16 (2025) 6348-6380

PMID: 40640160

Related EMDB entry:
Deposited:
2026-04-07
Released:
2026-04-21
Last modified:
2026-04-21
Imageset size:
457.31 GB
Imageset DOI:
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies in this entry were compressed by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

data/12122022/*.mrc:
  Compression was carried out by separating (“un-normalizing”) the gain.
  The details of the method are described in
  https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.
  
  Please apply the gain reference (data/12122022/gain-reference.mrc) during processing.

data/2022*.mrc:
  Compression was carried out in the same way as above.
  
  Please apply the gain reference (data/gain-reference.mrc) during processing.

data/2024*.mrc:
  Although the original movies are in float32 MRC, the pixel values are integers and
  apparently gain non-normalized. They were compressed by BZIP2 as is.
  Do NOT apply `gain-reference.mrc` above.

Original size:   4,154,835,504 kilobytes
Compressed size:   479,540,124 kilobytes
Contains:
  • micrographs - multiframe
1. alpha hemolysin heptamer in presence of Egg-PC/Chol liposomes
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
3843
Image size:
(None, None)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.92, 0.92)
Details:
---
Files:
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