CryoEM movies of Azotobacter vinelandii MoFeP determined using the SPT Labtech chameleon in the presence of 1x SurfACT. [multiple data sets in EER and MRC formats]
Publication:
A Surfactant Cocktail Overcomes Air-Water Interface Artifacts in Single-Particle CryoEM
Unaligned multi-frame micrographs of Azotobacter vinelandii MoFeP determined using the SPT Labtech chameleon in the presence of 1x SurfACT collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.
Aligned micrographs of Azotobacter vinelandii MoFeP determined using the SPT Labtech chameleon in the presence of 1x SurfACT collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were aligned in cryoSPARC.
Gain file required for motion correction of unaligned micrographs of Azotobacter vinelandii MoFeP determined using the SPT Labtech chameleon in the presence of 1x SurfACT.