- Microscope: FEI TITAN Krios - Detector: Gatan K3 - Acceleration voltage (kV): 300 - Average electron dose per image (e-/A2): 50 - Nominal Defocus (μm): 0.5–2.5 - Spherical aberration (Cs) : 2.7 - The pixel size in the image header (1 Å/px) is wrong. 0.8285 Å/px is the calibrated value. - Please use 'gain.tiff' as a gain reference without rotation and y-flipping.