EMPIAR-13420
SBF-SEM and FIB-SEM datasets and models of human macrophage and A431 cells [multiple data sets in TIFF format]
Publication:

Nuclear envelope-associated lipid droplets are enriched in cholesteryl esters and increase during inflammatory signaling

Szkalisity Á, Vanharanta L, Saito H, Vörös C, Li S, Isomäki A, Tomberg T, Strachan C, Belevich I, Jokitalo E, Ikonen E

The EMBO journal 44 (2025) 2774-2802

PMID: 40195500

Related EMDB entry:
Deposited:
2026-03-25
Released:
2026-06-01
Last modified:
2026-06-01
Imageset size:
37.87 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • reconstructed volumes
1. Focused ion beam scanning electron microscopy dataset of a differentiated primary human macrophage treated with tumor necrosis factor alpha (TNFa) for 1 h.
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
2719
Image size:
(4927, 785)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(60.0, 60.0)
Details:
Voxels: 0.006x0.006x0.012 um
Dwell time: 2us x2 line average
1.6kV, 1nA, EsB detector
Files:
Loading...
2. Focused ion beam scanning electron microscopy dataset of A primary human macrophage grown in control differentiation medium.
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
2888
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(60.0, 60.0)
Details:
Voxels: 0.006x0.006x0.012 um
Dwell time: 2us x2 line average
1.6kV, 1nA, EsB detector
Files:
Loading...
3. Serial block face scanning electron microscopy dataset of An A431 cell grown in complete medium
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
162
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(150.0, 150.0)
Details:
Voxels: 0.015x0.015x0.030 um
Dwell time: 10 us
2.5kV, spot size 3, Pressure: 0.22 Torr, WD = 5.7 mm
Files:
Loading...
4. Serial block face scanning electron microscopy dataset of A differentiated  primary human macrophage loaded with 200 μM cholesterol and 200 μM oleic acid for 2 hours.
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
143
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(150.0, 150.0)
Details:
Voxels: 0.015x0.015x0.030 um
Dwell time: 10 us
2.5kV, spot size 3, Pressure: 0.30 Torr, WD = 5.7 mm
Files:
Loading...
5. Serial block face scanning electron microscopy dataset of A differentiated  primary human macrophage loaded with 200 μM cholesterol and 200 μM oleic acid for 2 hours.
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
154
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(100.0, 100.0)
Details:
Voxels: 0.010x0.010x0.030 um
Dwell time: 5 us
2.5kV, spot size 3, Pressure: 0.22 Torr, WD = 5.7 mm
Files:
Loading...
6. Serial block face scanning electron microscopy dataset of A differentiated  primary human macrophage loaded with 200 μM cholesterol and 200 μM oleic acid for 2 hours.
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
207
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(150.0, 150.0)
Details:
Voxels: 0.015x0.015x0.030 um
Dwell time: 8 us
2.5kV, spot size 3, Pressure: 0.22 Torr, WD = 5.64 mm
Files:
Loading...
7. Serial block face scanning electron microscopy dataset of A primary human macrophage grown in control differentiation medium.
Category:
reconstructed volumes
Image format:
TIFF
No. of images or tilt series:
257
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(100.0, 100.0)
Details:
Voxels: 0.010x0.010x0.030 um
Dwell time: 5 us
2.5kV, spot size 3, Pressure: 0.22 Torr, WD = 5.64 mm
Files:
Loading...
He L, Bartesaghi A. (2026)
Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)
Kong L, Zottig X, Elferich J, Grigorieff N. (2026)
Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)
Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)
Jones HN, Deshmukh A, Pande K. (2026)
Gyawali R, Dhakal A, Wang L, Cheng J. (2026)
Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)
Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)
Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)