- Microscope: CRYO ARM™ 200 - Detector: Gatan K3 (6k × 4k) - Acceleration voltage (kV): 200 - Spherical aberration (Cs): 2.7 - Nominal Defocus (nm): -700 to -2200 - Average electron dose per image (e-/A2): 40 - Please use "CountCDSRef_ScGlgP_AMP_47-1_0000_Oct10_17.01.17.mrc" as a gain reference without rotation or flipping in cryoSPARC.