EMPIAR-13370 - Clostridium butyricum Argonaute wild type protein in binary form (single stranded guide DNA bound) [18989 multi-frame micrographs composed of 60 frames each in EER format]

EMPIAR-13370

Clostridium butyricum Argonaute wild type protein in binary form (single stranded guide DNA bound) [18989 multi-frame micrographs composed of 60 frames each in EER format]

Publication:

Target-DNA binding-induced dimerization is required for catalytic activation of Clostridium butyricum Argonaute

Barendse P, van Drimmelen M, Pacesa M, Sivaraman A, Zhang DK, Nickel L, Niault T, Lindhoud S, Roosjen M, Hegge JW, Park K, Correia BE, van der Oost J, Joo C, Westphal AH, Swarts DC

(2026)

Related EMDB entry:

Deposited:

2026-03-14

Released:

2026-03-17

Last modified:

2026-03-17

Imageset size:

4.08 TB

Imageset DOI:

10.6019/EMPIAR-13370

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Movies of CbAgo wild type in binary form, complexed with guide DNA

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

18989

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.574, 0.574)

Details:

Sample of wild type Clostridium butyricum Argonaute in binary form, bound to single stranded guide DNA ([P]-ATTTATGTGATTTTTC). Purified in buffer 20 mM Tris-HCl pH 7.5, 250 mM KCl, 5% glycerol.
60 frame movies, with a 50 e/A^2 electron dose, on a 300 kV Titan Krios with Falcon 4i detector, with 2.7 mm C2 lens, collected with a 0.574 A pixel size.

Files:

このエントリはPDBjからダウンロードすることはできません。

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

ダウンロードするファイル数やサイズによっては、膨大な時間がかかります。また、この機能は不安定で正常に動作しない可能性があり、ダウンロードされたファイルのチェックサムは検証されません。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Browse EMPIAR

Sample Preparation Widget

Volume Browser

Tomo Viewer Prototype

Claim entries to your ORCiD

Talks and Tutorials

EMPIAR Quick tour

Publications

Re-use study

Statistics

EMPIAR in the news

COVID-19 Data Portal

EMDB

BioImage Archive

PDBe

EMNavigator@PDBj

PDBj

Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)