Solubilized octameric pore of actinoporin Fav prepared on DOPC:sphingomyelin membranes [2073 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:
Cryo-EM structures of a protein pore reveal a cluster of cholesterol molecules and diverse roles of membrane lipids
The movies were imaged (Titan Krios, 300 kV) in counting mode with K2 Summit (Gatan) direct electron detector at a pixel size of 1.043 Å with a total electron dose of 40 e-/Å2.