EMPIAR-12993
Single-particle cryo-EM dataset of H. neapolitanus CsoSCA C283A/C284A inactive mutant [multiple data sets in TIFF and MRC formats]
Publication:

Single-particle cryo-EM dataset of H. neapolitanus CsoSCA C283A/C284A inactive mutant

Vogiatzi N, Gaullier G, Blikstad C

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Related EMDB entry:
Deposited:
2025-09-17
Released:
2026-05-01
Last modified:
2026-05-01
Imageset size:
1.70 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
1. Raw movies
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
10000
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.648, 0.648)
Details:
The gain reference needs to be applied: gainref_counted.mrc (no flip nor rotation needed in CryoSPARC).

Particle coordinates are provided as .cs files from CryoSPARC, also converted to .star files using pyem (see README file for details).

J40_particles_exported: coordinates of hexamer particles
J61_particles_exported: coordinates of dimer sub-particles (after C3 symmetry expansion and 3D classification), state 1
J65_particles_exported: coordinates of dimer sub-particles (after C3 symmetry expansion and 3D classification), state 2
Files:
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2. Gain reference file
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(None, None)
Details:
Gain reference file.
Files:
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