EMPIAR-12960 - Cryo-EM micrographs of R2Tg retrotransposon initiating target-primed reverse transcription [10628 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12960

Cryo-EM micrographs of R2Tg retrotransposon initiating target-primed reverse transcription [10628 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Structure and biochemistry-guided engineering of an all-RNA system for DNA insertion with R2 retrotransposons

Edmonds KK, Wilkinson ME, Strebinger D, Chen H, Lash B, Schaefer CC, Zhu S, Liu D, Zilberzwige-Tal S, Ladha A, Walsh ML, Frangieh CJ, Vaz Reay NA, Macrae RK, Wang X, Zhang F

Nature communications 16 (2025)

PMID: 40603868

DOI: 10.1038/s41467-025-61321-z

Related EMDB entry:

EMD-47091

Deposited:

2025-09-08

Released:

2025-11-30

Last modified:

2025-11-30

Imageset size:

1.68 TB

Imageset DOI:

10.6019/EMPIAR-12960

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of Taeniopygia guttata R2 retrotransposon initiating target-primed reverse transcription

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

10628

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.663, 0.663)

Details:

RAW MICROGRAPHS AND REFINEMENT STAR FILE FOR R2Tg TPRT COMPLEX (EMD-47091; PDB 9DOU)
Data collected at MIT.nano on Thermo Scientific Titan Krios G3i cryo-TEM

Voltage 300 keV
Camera Gatan K3, super-resolution mode with 2-fold binning by EPU
Energy filter GIF, 20eV slit width
Magnification 130000
Pixel size 0.663 A/pix
Dose 59.6 e/A2
Exposure rate 25.7 e/pix/s
Exposure time 1.02 s
Fractions 40

Total mics 10628

GainReference.mrc was binned using 'rebin by two' in Digital Micrograph. It needs to be flipped for use in RELION motion correction (Gain flip: Flip upside down (1) ). It can probably be used as is for cryoSPARC.

particles.star contains metadata for the particle selected for the final reconstruction.

Files:

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