EMPIAR-12847 - Human polymerase epsilon bound to PCNA and DNA with a pre-existing mismatch in the blocked conformation [18426 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12847

Human polymerase epsilon bound to PCNA and DNA with a pre-existing mismatch in the blocked conformation [18426 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

The proofreading mechanism of the human leading-strand DNA polymerase ε holoenzyme

Wang F, He Q, O'Donnell ME, Li H

Proceedings of the National Academy of Sciences of the United States of America 122 (2025)

PMID: 40440070

DOI: 10.1073/pnas.2507232122

Related EMDB entry:

EMD-49302 EMD-49303

Deposited:

2025-06-16

Released:

2025-09-09

Last modified:

2025-09-09

Imageset size:

10.76 TB

Imageset DOI:

10.6019/EMPIAR-12847

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. unaligned multi-frame micrographs of Human polymerase epsilon bound to PCNA and DNA with a pre-existing mismatch

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

18426

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.414, 0.414)

Details:

The micrographs are the average, without any realigment, of 50 raw movie frames (accumulating 60 electron per squared Angstrom in a 1.0 second exposure).

Files:

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