EMPIAR-12844 - E. coli 70S ribosome complex (N1-methylated 16S A1408 + G418) [5302 multi-frame micrographs composed of 29 frames each in TIFF format]

EMPIAR-12844

E. coli 70S ribosome complex (N1-methylated 16S A1408 + G418) [5302 multi-frame micrographs composed of 29 frames each in TIFF format]

Publication:

Basis for selective drug evasion of an aminoglycoside-resistance ribosomal RNA modification

Mattingly JM, Dey D, Zelinskaya N, Dunham CM, Conn GL

(---)

Related EMDB entry:

EMD-44194

Deposited:

2025-06-05

Released:

2025-10-27

Last modified:

2025-10-27

Imageset size:

3.70 TB

Imageset DOI:

10.6019/EMPIAR-12844

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of E. coli 70S ribosome complexes containing P-site tRNA-fMet, A-site tRNA-Val, N1-methylated 16S nucleotide A1408, and G418

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5302

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.814, 0.814)

Details:

Micrographs are gain-corrected multi-frame TIFF movies collected using a Thermo Fisher Titan Krios operating at 300 kV and a Thermo Fisher Falcon 4i direct electron detector. Images were collected with a total accumulated dose of 50 electrons per square angstrom. Image filenames terminate in "Fractions.tif". Files whose names terminate in "Fractions.xml" contain EPU-format image shift data that can be used for image shift group splitting.

Files:

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