EMPIAR-12833 - Cryo-electron microscopy of virus-like particle composed of PVY coat protein with A161R mutation [900 multi-frame micrographs composed of 37 frames each in TIFF format]

EMPIAR-12833

Cryo-electron microscopy of virus-like particle composed of PVY coat protein with A161R mutation [900 multi-frame micrographs composed of 37 frames each in TIFF format]

Publication:

Species-specific structural adaptation of the potyviral coat protein in virions and virus-like particles

Koritnik N, Kežar A, Kavčič L, Tušek Žnidarič M, Leonardi A, Pollari M, Mäkinen K, Podobnik M

(---)

DOI: 10.1038/s42003-025-09502-w

Related EMDB entry:

Deposited:

2025-05-22

Released:

2026-01-15

Last modified:

2026-01-15

Imageset size:

634.13 GB

Imageset DOI:

10.6019/EMPIAR-12833

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of PVY VLP with CP mutation A161R

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

900

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.95, 0.95)

Details:

The movies were imaged (Glacios, 200 kV) in counting mode with Falcon3 (Thermo Fisher Scientific) direct electron detector at a nominal magnification of 150,000 with a total electron dose of 40 e-/Å2.

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Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

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Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

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Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)