EMPIAR-12832 - Cryo-electron microscopy of virus-like particle composed of PVY coat protein with Q87H and R208T mutations [1422 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12832

Cryo-electron microscopy of virus-like particle composed of PVY coat protein with Q87H and R208T mutations [1422 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Species-specific structural adaptation of the potyviral coat protein in virions and virus-like particles

Koritnik N, Kežar A, Kavčič L, Tušek Žnidarič M, Leonardi A, Pollari M, Mäkinen K, Podobnik M

(---)

DOI: 10.1038/s42003-025-09502-w

Related EMDB entry:

Deposited:

2025-05-22

Released:

2026-01-15

Last modified:

2026-01-15

Imageset size:

1.05 TB

Imageset DOI:

10.6019/EMPIAR-12832

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of PVY VLP with CP mutations Q87H and R208T

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1422

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.95, 0.95)

Details:

The movies were imaged (Glacios, 200 kV) in counting mode with Falcon3 (Thermo Fisher Scientific) direct electron detector at a nominal magnification of 150,000 with a total electron dose of 40 e-/Å2.

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Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

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Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)