EMPIAR-12722 - Structural insights into transcriptional regulation by the helicase RECQL5 [9048 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12722

Structural insights into transcriptional regulation by the helicase RECQL5 [9048 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structural insights into transcriptional regulation by the helicase RECQL5

Florez Ariza AJ, Lue NZ, Grob P, Kaeser B, Fang J, Kassube SA, Nogales E

bioRxiv (2025)

PMID: 39975028

DOI: 10.1101/2025.01.29.634372

Related EMDB entry:

EMD-48076

Deposited:

2025-04-22

Released:

2025-05-30

Last modified:

2025-05-30

Imageset size:

2.45 TB

Imageset DOI:

10.6019/EMPIAR-12722

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of human RNA Polymerase II elongation complex bound to the RECQL5 helicase in the presence of ADP

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9048

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.048, 1.048)

Details:

This dataset contains raw, uncorrected movies collected in non-CDS, non-super-resolution mode (collected using a Titan Krios G3i, 300 kV, spherical aberration 2.7 mm, total dose 50 e/A^2). The pixel size is 1.048 A. The directory also contains the gain reference file (CountRef_240917_RECQL5-ADP_176-1_0001_X-2Y+0-1.mrc). This should not be rotated or flipped for processing in cryoSPARC.

Files:

このエントリはPDBjからダウンロードすることはできません。

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

ダウンロードするファイル数やサイズによっては、膨大な時間がかかります。また、この機能は不安定で正常に動作しない可能性があり、ダウンロードされたファイルのチェックサムは検証されません。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Browse EMPIAR

Sample Preparation Widget

Volume Browser

Tomo Viewer Prototype

Claim entries to your ORCiD

Talks and Tutorials

EMPIAR Quick tour

Publications

Re-use study

Statistics

EMPIAR in the news

COVID-19 Data Portal

EMDB

BioImage Archive

PDBe

EMNavigator@PDBj

PDBj

Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)