EMPIAR-12721 - Structural insights into transcriptional regulation by the helicase RECQL5 [12100 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12721

Structural insights into transcriptional regulation by the helicase RECQL5 [12100 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structural insights into transcriptional regulation by the helicase RECQL5

Florez Ariza AJ, Lue NZ, Grob P, Kaeser B, Fang J, Kassube SA, Nogales E

bioRxiv (2025)

PMID: 39975028

DOI: 10.1101/2025.01.29.634372

Related EMDB entry:

EMD-48075

Deposited:

2025-04-22

Released:

2025-05-30

Last modified:

2025-05-30

Imageset size:

3.18 TB

Imageset DOI:

10.6019/EMPIAR-12721

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of human RNA Polymerase II elongation complex bound to the RECQL5 helicase in the presence of AMPPNP

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

12100

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.048, 1.048)

Details:

This dataset contains raw, uncorrected movies collected in non-CDS, non-super-resolution mode (collected using a Titan Krios G3i, 300 kV, spherical aberration 2.7 mm, total dose 50 e/A^2). The pixel size is 1.048 A. The directory also contains the gain reference file (CountRef_04092024_RECQL5_AMPPNP_161_0001_X+0Y+0-1.mrc 89.9 MB). This should not be rotated or flipped for processing in cryoSPARC.

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