- Microscope: FEI TITAN Krios - Acceleration voltage (kV): 300 - Spherical aberration (Cs): 2.7 - Nominal Defocus (nm): -800 to -1800 - Detector: Gatan K3 BIOQUANTUM - Average electron dose per image (e-/A2): 50 - Please use gain.mrc as a gain reference. - In CryoSPARC, select "flip gain ref in Y".