- Microscope: CRYO ARM™ 200 - Acceleration voltage (kV): 200 - Pixel size (Å/pix): 0.83 - Spherical aberration (Cs): 2.7 - Nominal Defocus (nm): -700 to -2200 - Detector: Gatan K3 (6k × 4k) - Average electron dose per image (e-/A2): 40 - Number of frames per image: 40 - Please use gain.mrc as a gain reference without rotation or flipping in cryoSPARC.