Pixel size for Cell 1 is 6nm with a total of 3598 frames/slices. Cell 2 and Cell 3 are caputred at 5nm resolution with 3280 and 2659 frames respectively. All stacks were aligned and cropped. For segmentation, voxel type was reduced to 8 bits and further binned if necessary (Indicated in supplementary table S3 of the article)
Pixel size for Cell 1, 2 and 3 is 8nm with 695, 1874 and 2244 frames respectively. All stacks were aligned, cropped and converted from 16 bit to 8 bit voxel type. Cell 1 and 3 are inverted. Any binning done during segmentation are indicated in supplementary table S3 of the article
All cells were captured with a 6nm resolution with about 660-755 frames. All stacks were aligned and cropped. Cells 4 and 5 are inverted and converted to 8 bit voxel type while cells 1-3 remain as 16 bit voxel type. For segmentation, voxel type for all cells was reduced to 8 bits
All cells were captured with a 6nm resolution with about 462-688 frames. All stacks were aligned and cropped. Cells 4-7 are inverted and converted to 8 bit voxel type while cells 1-3 remain as 16 bit voxel type. For segmentation, voxel type was reduced to 8 bits for all cells