EMPIAR-12596 - Cryo-EM of SARS-CoV-2 nsp13-replication-transcription complex (nsp13-RTC) [4358 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12596

Cryo-EM of SARS-CoV-2 nsp13-replication-transcription complex (nsp13-RTC) [4358 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex

Chen J, Malone B, Llwewllyn E, Grasso M, Shelton PMM, Olinares PDB, Maruthi K, ENG ET, Vatandaslar H, Chait BT, Kapoor TM, Darst SA, Campbell EA

Cell 182 (2020) 1560-1573

PMID: 32783916

DOI: 10.1016/j.cell.2020.07.033

Related EMDB entry:

EMD-22270 EMD-22271 EMD-22160

Deposited:

2025-02-23

Released:

2025-04-09

Last modified:

2025-04-09

Imageset size:

1.29 TB

Imageset DOI:

10.6019/EMPIAR-12596

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4358

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.1, 1.1)

Details:

FEI Titan Krios + Gatan K3, counting mode, BioQuantum energy filter 20 eV slit, Cs 2.7 mm; accelerating voltage 300 kV; dose rate 24.7 e-/ A^2/s, frame time 0.05 s (50 frames) with total exposure of 2.5 s, total dose of 64 e-/A^2; pixel size 1.1 A/px(detector); defocus range -0.8 to -2.5 um; for gain reference, see data/rawdata/references/reference_list.txt.

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Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

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Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)