Raw unaligned frames were preprocessed in Warp to generate tilt series stacks. Corresponding count reference files (mrc file) are in the subfolders of the EMPIAR_upload/Frames folder. The number of frames per image varies from 38 to 42. Subfolder names with YTC041_1, YTC041_2, and YTC042_2 are datasets acquired on S. cerevisiae grown in YPG. Subfolder names with YTC043_1 and YTC043_2 are datasets acquired on S. cerevisiae grown in YPD.
The tilt series stacks (.st file) and rawtlt files (in Tilt_series_rawtlt) were created from Warp with the input of tilt-corrected mdoc files. Raw mdoc files are listed in the EMPIAR_upload/PACE_mdoc folder. Tilt series stacks are then aligned in IMOD.
Bin6 tomograms are reconstructed from Warp after IMOD alignment. The coordinates of 80S ribosome and co-translating ribosome are provided in the EMPIAR_upload/Particle_lists folder.
Raw unaligned frames were preprocessed in Warp to generate tilt series stacks. Corresponding count reference files (mrc file) are in the subfolders of the EMPIAR_upload_nonCHX/Frames folder. The number of frames per image varies from 39 to 41. Subfolder name with YTC009_3 is the dataset acquired on S. cerevisiae grown in YPG, and subfolder name with YTC025_2 is the dataset acquired on S. cerevisiae grown in YPD.
The tilt series stacks (.st file) and rawtlt files (in EMPIAR_upload_nonCHX/Tilt_series_rawtlt) were created from Warp with the input of tilt-corrected mdoc files. Raw mdoc files are listed in the EMPIAR_upload_nonCHX/mdocs folder. Tilt series stacks are then aligned in IMOD.
Bin6 tomograms are reconstructed from Warp after IMOD alignment. The coordinates of 80S ribosome and co-translating ribosome are provided in the EMPIAR_upload_nonCHX/Particle_lists folder.
