EMPIAR-12510
Mouse LRRC8A(BRIL):D in GDN [multiple data sets in TIFF, MRCS and MRC formats]
Publication:

Assembly and lipid-gating of LRRC8A:D volume-regulated anion channels

Lurie A, Kern DM, Henn K, Brohawn SG

bioRxiv (2024)

Related EMDB entry:
Deposited:
2024-12-03
Released:
2025-01-21
Last modified:
2025-01-21
Imageset size:
3.01 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - multiframe - processed
1. Unaligned tif movies for LRRC8A(BRIL):D
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
9730
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.048, 1.048)
Details:
Gain reference in .mrc form is included in the folder with the movies (data/Frames/). In RELION, the gain must be flipped upside down (1).
Files:
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2. Complete polished particles stack for LRRC8A(BRIL):D
Category:
picked particles - multiframe - processed
Image format:
MRCS
No. of images or tilt series:
8105
Image size:
(416, 416)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.048, 1.048)
Details:
Contains all polished particles (220,293) as 8,105 .mrcs files. The particle information is in the main data folder in RELION .star format (data/AllPolishedParticles.star). The particles can also be imported into cryoSPARC for processing. Note that this is the full polished particles stack, which was processed further in cryoSPARC to clean the stack and to separate the two conformations.
Files:
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3. Particles stack for LRRC8A(BRIL):D conformation 1
Category:
picked particles - multiframe - processed
Image format:
MRC
No. of images or tilt series:
43907
Image size:
(416, 416)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.048, 1.048)
Details:
Final particles stack for LRRC8A(BRIL):D conformation 1, restacked from cryoSPARC.
Files:
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4. Particles stack for LRRC8A(BRIL):D conformation 2
Category:
picked particles - multiframe - processed
Image format:
MRC
No. of images or tilt series:
46893
Image size:
(416, 416)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.048, 1.048)
Details:
Final particles stack for LRRC8A(BRIL):D conformation 2, restacked from cryoSPARC.
Files:
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