EMPIAR-12490 - Single particle reconstruction of LGTV with prME from TBEV [32123 multi-frame micrographs composed of 1314 frames each in EER format]

EMPIAR-12490

Single particle reconstruction of LGTV with prME from TBEV [32123 multi-frame micrographs composed of 1314 frames each in EER format]

Publication:

The influence of the pre-membrane and envelope proteins on structure, pathogenicity and tropism of tick-borne encephalitis virus

Rosendal E, Bisikalo K, Willekens SM, Lindgren M, Holoubek J, Svoboda P, Lappalainen A, Könighofer E, Mirgorodskaya E, Nordén R, Morini F, Rosenbaum W, Růžek D, Ahlgren U, Anastasina M, Merits A, Butcher SJ, Nilsson E, Överby AK

bioRxiv (2024)

DOI: 10.1101/2024.10.16.618642

Related EMDB entry:

EMD-50518

Deposited:

2024-06-19

Released:

2025-01-29

Last modified:

2025-01-29

Imageset size:

16.81 TB

Imageset DOI:

10.6019/EMPIAR-12490

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned EER movies of the purified chimeric LGTV(TBEV:prME)

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

32123

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.917, 0.917)

Details:

Micrographs collected of a Titan Krios 300 keV, equipped with a Falcon 4i. Spherical aberration 2.7, total dose 40. Gain reference in the main data folder. When building atomic model had scale issues, recalibrated voxel size of the map to 0.944 angstrom. CryoSPARC workflow available in the main data folder.

Files:

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Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)