EMPIAR-12447 - Cryo-EM Structure of the Full-length hnRNPA1 Amyloid Fibril [5652 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12447

Cryo-EM Structure of the Full-length hnRNPA1 Amyloid Fibril [5652 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cryo-EM Structure of the Full-length hnRNPA1 Amyloid Fibril

Sharma K, Banerjee S, Savran D, Rajes C, Wiese S, Girdhar A, Schwierz N, Lee C, Shorter J, Schmidt M, Guo L, Fändrich M

Journal of molecular biology 435 (2023)

PMID: 37481159

DOI: 10.1016/j.jmb.2023.168211

Related EMDB entry:

EMD-14739

Deposited:

2024-11-13

Released:

2025-06-02

Last modified:

2025-06-02

Imageset size:

928.00 GB

Imageset DOI:

10.6019/EMPIAR-12447

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of full-length hnRNPA1 amyloid fibrils

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5652

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.04, 1.04)

Details:

Krios1 Quantum-K2
Cs 2.7
300kV EFTEM nanpprobe
x130k spot 8 beam size 620nm
c2=50 objective_aperture=100

GIF-K2 counting
12s exposure 40 frames
total dose at the sample 44.90e/A2
1 pixel = 1.04A
dose rate ~3 e/pix/s
defocus range -1.2 to -2.5

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Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

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Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)